Product Name: HIWI Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P
Applications: ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1μg/ml antibody staining. Please note that currently we cannot find an explanation in the Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration, 4-6μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: HIWI antibody was raised against a 13 amino acid synthetic peptide near the internal region of HIWI.
Host Species: Goat
Purification: HIWI antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1802326-66-4
Product: SR12813
Buffer: HIWI antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: PIWIL1, HIWI, PIWI, piwi-like 1 (Drosophila), piwi (Drosophila)-like 1, homolog of Drosophila piwi
Accession NO.: NP_004755.2
Protein Ino: 66346725
Official Symbol: PIWIL1
Geneid: 9271
Background:
PubMed ID:http://aac.asm.org/content/22/6/1067.abstract
