Product Name: Histone H2A.X (phospho Ser139) Antibody
Species Reactivity: Human
Tested Applications: IF, WB
Applications: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 15 kDa
Immunogen: Histone H2A.X (Phospho-Ser139) antibody was raised against a peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Physical State: Liquid
CAS NO.: 115103-85-0
Product: GSK2256098
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: H2AX, H2A.X, H2A/X, HNF3B, TCF3B, H2AFX, H2a/x, HIST5-2AX
Accession NO.: NP_002096.1
Protein Ino: 4504253
Official Symbol: H2AFX
Geneid: 3014
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
PubMed ID:http://aac.asm.org/content/22/5/889.abstract
