Product Name: Histone H2A.X (Ab 139) Antibody
Species Reactivity: Human
Tested Applications: IF, IHC, WB
Applications: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 15 kDa
Immunogen: Histone H2A.X (Ab-139) antibody was raised against a peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 1113-59-3
Product: AM-2394
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: H2AX, H2A.X, H2A/X, MCH6, H2AFX, H2a/x, HIST5-2AX
Accession NO.: NP_002096.1
Protein Ino: 4504253
Official Symbol: H2AFX
Geneid: 3014
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
PubMed ID:http://aac.asm.org/content/22/5/882.abstract
