Product Name: HER2 Antibody [2KRI7]
Species Reactivity: Human
Tested Applications: Flow, IF, IHC
Applications: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at Room Temp (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant human protein was used as the immunogen for this HER2 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1219810-16-8
Product: S49076
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: erbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu, MLN19, NEU, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Accession NO.:
Protein Ino:
Official Symbol: Her2
Geneid: 2064
Background: HER2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. [RefSeq]
PubMed ID:http://aac.asm.org/content/22/1/176.abstract
