Product Name: Heparanase Antibody
Species Reactivity: Human, Mouse
Tested Applications: IHC, WB
Applications: Heparanase antibody can be used in ELISA, Western Blot starting at 1:500 – 1:1000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Heparanase antibody was raised against 50kD – 8kD Heparanase heterodimer (Human).
Host Species: Rabbit
Purification: Protein G Column
Physical State: Liquid
CAS NO.: 1118567-05-7
Product: Thiomyristoyl
Buffer: PBS, pH 7.2, 0.01% Thimerosal
Concentration:
Storage Conditions: Store Heparanase antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HPSE, Endo-glucoronidase, Heparanase-1, Heparanase, HPA, HSE1, HEP, HPA1, HPSE1, HPR1
Accession NO.: Q9Y251
Protein Ino: 296434532
Official Symbol: HPSE
Geneid: 10855
Background: Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50k D subunit harboring the active site and a 8kD subunit. It is produced as a latent 65 kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival. Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Reacts with the 65 kD precursor as well as the 50 kD and 8 kD subunits of human or mouse Heparanase.
PubMed ID:http://aac.asm.org/content/21/6/935.abstract
