Product Name: HCC-1 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hHCC-1 by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hHCC-1. Sandwich: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human HCC-1 (XP-5151) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hHCC-1.Western Blot:To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hHCC-1 (human HCC-1).
Host Species: Rabbit
Purification: Anti-hHCC-1 specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 125-65-5
Product: CEP-40783
Buffer:
Concentration:
Storage Conditions: HCC-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: CC-1, CC-3, CKB1, MCIF, NCC2, SY14, HCC-1, HCC-3, NCC-2, SCYL2, SCYA14, HCC-1(1-74), HCC-1/HCC-3, C-C motif chemokine 14,
Accession NO.: Q16627
Protein Ino: 2493670
Official Symbol: CCL14
Geneid: 6358
Background:
PubMed ID:http://aac.asm.org/content/21/3/390.abstract
