Product Name: GRP94 Antibody [SRPR90b]
Species Reactivity: Human
Tested Applications: IF, IHC-P, WB
Applications: Western blot: 1-2 ug/mlImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the GRP94 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.
Host Species: Rat
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 646995-35-9
SU 5402
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1
Accession NO.:
Protein Ino:
Official Symbol: HSP90B1
Geneid: 7184
Background: Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]
PubMed ID:http://aac.asm.org/content/20/2/267.abstract
