Product Name: GRO/KC Antibody
Species Reactivity: Rat
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat GRO/KC (100 ng/mL), a concentration of 5-10 μg/mL of this antibody is required. ELISA:To detect Rat GRO/KC by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant Rat GRO/KC. Sandwich: To detect Rat GRO/KC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GRO/KC (XP-5146Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat GRO/KC.Western Blot:To detect Rat GRO/KC by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GRO/KC is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat GRO/KC.
Host Species: Rabbit
Purification: Anti-Rat GRO/KC specific antibody was purified by affinity chromatography employing immobilized Rat GRO/KC matrix.
Physical State: Lyophilized
CAS NO.: 1157-39-7
CP-673451
Buffer:
Concentration:
Storage Conditions: GRO/KC antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Gro1, CINC-1, Cinc1, Gro, Scyb1, Growth-regulated alpha protein, C-X-C motif chemokine 1
Accession NO.: P14095
Protein Ino: 1170021
Official Symbol: Cxcl1
Geneid: 81503
Background:
PubMed ID:http://aac.asm.org/content/20/2/184.abstract
