Product Name: GRO-B Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hGRO-β by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hGRO-β. Sandwich:To detect hGRO-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human GRO-beta (XP-5148) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGRO-beta.Western Blot:To detect hGRO-β by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-β is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO-β (human GRO-β).
Host Species: Rabbit
Purification: Anti-hGRO-β specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 4460-86-0
CY5-SE
Buffer:
Concentration:
Storage Conditions: GRO-B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, GRO2, GROB, C-X-C motif chemokine 2, Growth-regulated protein beta, Gro-beta
Accession NO.: P19875
Protein Ino: 127085
Official Symbol: CXCL2
Geneid: 2920
Background:
PubMed ID:http://aac.asm.org/content/20/2/168.abstract