Product Name: CD57 Antibody [HNK-1 or Leu-7]
Species Reactivity: Human
Tested Applications: IHC-P, WB
Applications: Western blot: 2-4 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the CD57 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Human peripheral blood mononuclear cells were used as the immunogen for the CD57 antibody.
Host Species: Mouse
Purification: PEG precipitation
Physical State: Liquid
CAS NO.: 931706-15-9
Product: PPQ-102
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P, B3GAT1, GLCATP
Accession NO.:
Protein Ino:
Official Symbol: B3GAT1
Geneid: 27087
Background: Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
PubMed ID:http://aac.asm.org/content/35/1/164.abstract
