Product Name: BOULE Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P
Applications: ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32. Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: BOULE antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of BOULE.
Host Species: Goat
Purification: BOULE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 16208-51-8
Product: Dimesna
Buffer: BOULE antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BOLL, bol, boule-like (Drosophila), BOULE
Accession NO.: NP_932074.1, NP_149019.1
Protein Ino: 37574731
Official Symbol: BOLL
Geneid: 66037
Background:
PubMed ID:http://aac.asm.org/content/42/4/821.abstract
