Product Name: BMP-7 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect hBMP-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBMP-7.SandwichTo detect hBMP-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human BMP-7 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBMP-7. Western Blot:To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBMP-7. Human BMP-7 specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 15291-77-7
Product: Ginkgolide B
Buffer:
Concentration:
Storage Conditions: BMP-7 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: OP-1, OP1, Bone morphogenetic protein 7, Osteogenic protein 1, BMP-7
Accession NO.: P18075
Protein Ino: 115078
Official Symbol: BMP7
Geneid: 655
Background: BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair.
PubMed ID:http://aac.asm.org/content/42/4/942.abstract
