Product Name: BMI-1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, ICC, IF, WB
Applications: BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1.The immunogen is located within amino acids 140 – 190 of BMI-1.
Host Species: Rabbit
Purification: BMI-1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 150-38-9
Product: Ethylenediaminetetraacetic acid (trisodium salt)
Buffer: BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: BMI-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4
Accession NO.: P35226
Protein Ino: 22258801
Official Symbol: BMI1
Geneid: 648
Background: BMI-1 Antibody: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.
PubMed ID:http://aac.asm.org/content/42/4/762.abstract
