Product Name: Beclin-1 Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, ICC, IF, WB
Applications: Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 – 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1.The immunogen is located within the last 50 amino acids of Beclin-1.
Host Species: Rabbit
Purification: Beclin-1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 34444-01-4
Product: Cefamandole
Buffer: Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Beclin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein
Accession NO.: AAH10276
Protein Ino: 16307457
Official Symbol: BECN1
Geneid: 8678
Background: Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
PubMed ID:http://aac.asm.org/content/42/1/23.abstract
