Product Name: BDNF Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: ELISA:To detect hBDNF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hBDNF. Western Blot:To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor).
Host Species: Rabbit
Purification: Anti-hBDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
Physical State: Lyophilized
CAS NO.: 3562-84-3
Product: Benzbromarone
Buffer:
Concentration:
Storage Conditions: BDNF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ANON2, BULN2Brain-derived neurotrophic factor, Abrineurin, BDNF
Accession NO.: P23560
Protein Ino: 114900
Official Symbol: BDNF
Geneid: 627
Background: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimers and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.
PubMed ID:http://aac.asm.org/content/53/12/5163.abstract
