Product Name: BD-1 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect hBD-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-1. Sandwich:To detect hBD-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required..Western Blot:To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μL/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-1. Human BD-1 specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification: BD-1 specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 137-08-6
Product: D-Pantothenic acid (hemicalcium salt)
Buffer:
Concentration:
Storage Conditions: BD-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: BD1, HBD1, DEFB-1, DEFB101, BD1, Beta-defensin 1, Defensin, beta 1, BD-1
Accession NO.: P60022
Protein Ino: 38503374
Official Symbol: DEFB1
Geneid: 1672
Background:
PubMed ID:http://aac.asm.org/content/53/12/5155.abstract
