Product Name: BCMA Antibody [Vicky-1]
Species Reactivity: Human
Tested Applications: Flow, ICC
Applications: BCMA antibody can be used to recognize human BCMA by Flow Cytometry and immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5 μg/mL Vicky-1 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5×105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of monoclonal antibody to BCMA (Vicky-1). After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. This product is for research use only.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: BCMA (monoclonal Vicky-1) antibody was raised against recombinant human BCMA:Fc.
Host Species: Rat
Purification: BCMA (monoclonal Vicky-1) antibody was purified to 95% in SDS-PAGE.
Physical State: Liquid
CAS NO.: 477-43-0
Product: Dehydrocostus Lactone
Buffer: PBS containing 0.02% sodium azide
Concentration:
Storage Conditions: BCMA antibody can be stored at 4˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: BCM, BCMA, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein, BCM, CD269, TNFRSF13A
Accession NO.: Q02223
Protein Ino: 313104029
Official Symbol: TNFRSF17
Geneid: 608
Background:
PubMed ID:http://aac.asm.org/content/53/12/5145.abstract
