Product Name: Bcl-rambo Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo.The immunogen is located within amino acids 280 – 330 of Bcl-rambo.
Host Species: Rabbit
Purification: Bcl-rambo Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 4618-18-2
Product: Lactulose
Buffer: Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Bcl-rambo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Bcl-rambo Antibody: MIL1, BCL-RAMBO, Bcl2-L-13, MIL1, CD003, Bcl-2-like protein 13, Bcl-rambo
Accession NO.: AAH07658
Protein Ino: 14043326
Official Symbol: BCL2L13
Geneid: 23786
Background: Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.
PubMed ID:http://aac.asm.org/content/53/12/4968.abstract
