Product Name: B71 Antibody (biotin)
Species Reactivity: Mouse
Tested Applications: ELISA, WB
Applications: ELISA:To detect mB-71 by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mB-71. Western Blot:To detect mB-71 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mB-71 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mB-71 (murine B-71).
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 474972
Product: Naftidrofuryl (oxalate)
Buffer:
Concentration:
Storage Conditions: B71 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7
Accession NO.: P33681
Protein Ino: 461606
Official Symbol: CD80
Geneid: 941
Background:
PubMed ID:http://aac.asm.org/content/53/9/3734.abstract