Product Name: ATG3 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: ATG3 antibody can be used for detection of ATG3 by Western blot at 1 – 2 μg/mL. The ATG3 autophagy antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3.The immunogen is located within amino acids 180 – 230 of ATG3.
Host Species: Rabbit
Purification: ATG3 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 66-81-9
Product: Cycloheximide
Buffer: ATG3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: ATG3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ATG3 Antibody: APG3, APG3L, PC3-96, APG3-LIKE, APG3, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, APG3-like
Accession NO.: NP_071933
Protein Ino: 19526773
Official Symbol: ATG3
Geneid: 64422
Background: ATG3 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
PubMed ID:http://aac.asm.org/content/53/7/2949.abstract
