Product Name: ATF2 (phospho Ser 490 / 498) Antibody
Species Reactivity: Human
Tested Applications: IHC, WB
Applications: The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 74
Immunogen: ATF-2 (Ser 490/498) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 859212-16-1
Product: Bafetinib
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: HB16, CREB2, TREB7, CREB-2, CRE-BP1, CREBP1, Activating transcription factor 2, cAMP-dependent transcription factor ATF-2
Accession NO.: P15336
Protein Ino: 215274241
Official Symbol: ATF2
Geneid: 1386
Background: The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.
PubMed ID:http://aac.asm.org/content/53/6/2569.abstract
