Product Name: ARID1A Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: ARID1A antibody can be used for detection of ARID1A by Western blot at 1 – 2 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 133, 227, 251 kDa Observed: 130, 260 kDa
Immunogen: ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.The immunogen is located within amino acids 580 – 630 of ARID1A.
Host Species: Rabbit
Purification: ARID1A antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 844903-58-8
Product: GSK163090
Buffer: ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: ARID1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1
Accession NO.: NP_006006
Protein Ino: 21264565
Official Symbol: ARID1A
Geneid: 8289
Background: The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
PubMed ID:http://aac.asm.org/content/53/4/1386.abstract
