Product Name: Aquaporin 2 (phospho Ser261) Antibody
Species Reactivity: Bovine, Chicken, Dog, Human, Mouse, Rat
Tested Applications: IHC, WB
Applications: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 29/37
Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 522650-83-5
Product: SW044248
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: Aquaporin 2 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AQP-2, aquaporin-2, ADH water channel, AQP-2
Accession NO.: P34080
Protein Ino: 461529
Official Symbol: Aqp2
Geneid: 25386
Background: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).
PubMed ID:http://aac.asm.org/content/53/3/1132.abstract
