Product Name: APRIL Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hAPRIL by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hAPRIL. Western Blot:To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL (Human APRIL).
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 1235034-55-5
Product: A-1155463
Buffer:
Concentration:
Storage Conditions: APRIL antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: APRIL, CD256, TALL2, ZTNF2, TALL-2, TRDL-1, UNQ383/PRO715, APRIL, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand
Accession NO.: O75888
Protein Ino: 13124605
Official Symbol: TNFSF13
Geneid: 8741
Background: APRIL (A PRoliferation Inducing Ligand) a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. Members of the TNF superfamily regulate immune responses and induce apoptosis.
PubMed ID:http://aac.asm.org/content/53/3/1194.abstract
