Product Name: ApoM Antibody [8F12C6B8]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: Western Blot:1:500 – 1:2,000 IF:1:200 – 1:1,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification: Antibody is purified by protein A affinity chromatography.
Physical State:
CAS NO.: 117793
Product: Cucurbitacin I
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
Storage Conditions: ApoM monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: G3A, NG20, HSPC336, Protein G3a, Apo-M, G3a, apo-M
Accession NO.: O95445
Protein Ino: 17370872
Official Symbol: APOM
Geneid: 55937
Background: ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21 kDa),is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.
PubMed ID:http://aac.asm.org/content/53/3/1074.abstract