Product Name: ApoE3 Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect hApoE3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hApoE3.SandwichTo detect hApoE3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human ApoE3 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hApoE3. Western Blot:To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography and then biotinylated. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 315706-13-9
Product: 4EGI-1
Buffer:
Concentration:
Storage Conditions: ApoE3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: AD2, LPG, LDLCQ5Apolipoprotein EApo-E
Accession NO.: P02649
Protein Ino: 114039
Official Symbol: APOE
Geneid: 348
Background:
PubMed ID:http://aac.asm.org/content/53/3/1149.abstract
