Product Name: APEX1 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: APEX1 antibody can be used for detection of APEX1 by ELISA at 1:312500. APEX1 antibody can be used for detection of APEX1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 35 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APEX1.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 1000998-59-3
Product: BMS-687453
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store APEX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APEX1, APE, APX, APE1, APEN, APEX, HAP1, REF1
Accession NO.: NP_542379
Protein Ino: 18375503
Official Symbol: APEX1
Geneid: 328
Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5 to the AP site. This gene encodes the major AP endonuclease in human cells.
PubMed ID:http://aac.asm.org/content/53/2/593.abstract
