Product Name: APC8 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, ICC, WB
Applications: APC8 antibody can be used for detection of APC8 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8.The immunogen is located within amino acids 270 – 320 of APC8.
Host Species: Rabbit
Purification: APC8 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1627710-50-2
Product: LJH685
Buffer: APC8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: APC8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: APC8 Antibody: APC8, CUT23, ANAPC8, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8
Accession NO.: Q9UJX2
Protein Ino: 254763423
Official Symbol: CDC23
Geneid: 8697
Background: APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
PubMed ID:http://aac.asm.org/content/53/2/814.abstract
