Product Name: Alpha-tubulin Antibody (biotin)
Species Reactivity: Chicken, Human, Mouse, Rabbit, Rat, Zebrafish
Tested Applications: ELISA, WB
Applications: Biotin-Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 1 – 2 μg/ml.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 50 kDa Observed: 55 kDa
Immunogen: Biotin-Alpha-tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-tubulin
Host Species: Rabbit
Purification: Biotin-Alpha-tubulin antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1523406-39-4
Product: SAR405
Buffer: Biotin-Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Biotin-Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
Accession NO.: CAA25855
Protein Ino: 37492
Official Symbol: TUBA1A
Geneid: 7846
Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and -Actin, this antibody makes an excellent loading control in immunoblots.
PubMed ID:http://aac.asm.org/content/52/9/3424.abstract
