Product Name: alpha-Actinin-4 Antibody
Species Reactivity: Hamster, Human, Mouse, Pig, Rat
Tested Applications: IHC, IP, WB
Applications: Immunocytochemistry: (1:250- 1:1,600) . Immunohistochemistry: (1:250- 1:1,600) . Immunoprecipitation: (1:10) . Western Blot: (1:1,000-1:2,500) . Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.
Host Species: Rabbit
Purification: Antigen affinity purified.
Physical State: Liquid
CAS NO.: 11089-65-9
Product: Tunicamycin
Buffer: Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.
Concentration: 250 ug/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: F-actin Cross-linking Protein
Accession NO.: NP_004915
Protein Ino: 12025678
Official Symbol: ACTN4
Geneid: 81
Background: alpha-Actinin 4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).
PubMed ID:http://aac.asm.org/content/52/9/3216.abstract
