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HSP90β monoclonal antibody (K3701)
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The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents. Flow cytometric analysis human colon cancer Caco-2 cells analyzed by flow cytometry using isotype control antibody (left) or Hsp90 β mAb (K3701) (right).{{869998-49-2} MedChemExpress|{869998-49-2} Biological Activity|{869998-49-2} Description|{869998-49-2} supplier} Western blot analysis: Lane 1: MW marker, Lane 2: HeL a, Lane 3: PC-12, Lane 4: CHO-K1, Lane 5: HSP90 Native Protein , Lane 6: HSP90 alpha Recombinant Protein (negative control) , probed with HSP90 β mAb (K3701) Flow cytometric analysis human colon cancer Caco-2 cells analyzed by flow cytometry using isotype control antibody (left) or Hsp90 β mAb (K3701) (right).{{356559-20-1} site|{356559-20-1} Protocol|{356559-20-1} In Vitro|{356559-20-1} custom synthesis} Western blot analysis: Lane 1: MW marker, Lane 2: HeL a, Lane 3: PC-12, Lane 4: CHO-K1, Lane 5: HSP90 Native Protein , Lane 6: HSP90 alpha Recombinant Protein (negative control) , probed with HSP90 β mAb (K3701)
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| Alternative Name HSP84, Heat shock protein 90β | Application Flow Cytometry, WB | Application Notes Detects a band of ~90kDa by Western blot.PMID:28749637 | Clone K3701 | Formulation Liquid. In saline, pH 6.9, containing 0.1% sodium azide. | Host Mouse | Immunogen Recombinant human HSP90β. | Isotype IgM | Recommendation Dilutions/Conditions Flow Cytometry (10µg/ml)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Source Purified from hybridoma tissue culture supernatant. | Species Reactivity Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Sheep | UniProt ID P08238 | Unit of Measure (UM) µg
