Product Name :
Anti-GST, Rabbit antibody(Biotin)
Applications:
WB,ELISA,IP
Reactivity :
GST
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-GST, Rabbit antibody(Biotin) is designed for detecting GST fusion proteins specifically. Anti-GST, Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to GST coupled to Biotin, and Anti-GST, Rabbit antibody(Biotin) detects the GST selectively, no reactivity with other proteins. | | Immunogen: GST fusion protein | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc | Conjugate: Biotin | Specificity: GST | Cross-Reactivity: Highly selective for GST sequence | Affinity: Dissociation constant KD of 0.{{702674-56-4} medchemexpress|{702674-56-4} Purity & Documentation|{702674-56-4} In Vitro|{702674-56-4} custom synthesis} 1nM | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles) | Background:Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest.{{1610943-06-0} MedChemExpress|{1610943-06-0} Biological Activity|{1610943-06-0} Description|{1610943-06-0} custom synthesis} Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade.PMID:29697234 GST is one useful epitope ta for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Description2 :
Anti-GST, Rabbit antibody(Biotin) is designed for detecting GST fusion proteins specifically. Anti-GST, Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to GST coupled to Biotin, and Anti-GST, Rabbit antibody(Biotin) detects the GST selectively, no reactivity with other proteins.
Immunogen:
GST fusion protein
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Store at –20 °C(Avoid freeze / thaw cycles)
Function:
WB: 1:5,000-1:20000 ELISA: 1:5,000-1:20000IP: 1-2ug/sampleDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
