anti-histone h2a.x mouse mab

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Anti-Histone H2A.X Mouse mAb Clone Number: 2H8 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Histone H2AX, H2a/x, H2AX, H2AFX Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H2AX, H2a/x, H2AX, H2AFX UniProt ID P16104 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2A.X protein MW (kDa) 15 Specificity Anti-Histone H2A.X Mouse mAb detects endogenous levels of histone H2A.X protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Among core histones, the H2A family shows the highest sequence divergence, especially in the C-terminal region, leading to variants including H2A.Z, H2A.X, MacroH2A, H2A.B etc. H2A histone family member X (H2A.X) is a histone H2A variant encoded by the H2AFX gene. H2A.X contributes to nucleosome-formation, chromatin-remodeling and DNA double-strand break repair and is considered as a tumor suppressor. An early cellular response to DNA damage is the rapid phosphorylation of H2A.X at position Ser139. Cellular location Nucleus Images WB Lysate: MCF-7, HeLa, H1299, JKProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 100 mg/ml nocodazole for 18 h and 100 nM calyculin A for 1 hProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa :
Anti-Histone H2A.X Mouse mAb Clone Number: 2H8 Host: Mouse Clonality: Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Histone H2AX, H2a/x, H2AX, H2AFX Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H2AX, H2a/x, H2AX, H2AFX UniProt ID P16104 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2A.X protein MW (kDa) 15 Specificity Anti-Histone H2A.X Mouse mAb detects endogenous levels of histone H2A.X protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Among core histones, the H2A family shows the highest sequence divergence, especially in the C-terminal region, leading to variants including H2A.Z, H2A.X, MacroH2A, H2A.B etc. H2A histone family member X (H2A.X) is a histone H2A variant encoded by the H2AFX gene. H2A.X contributes to nucleosome-formation, chromatin-remodeling and DNA double-strand break repair and is considered as a tumor suppressor. An early cellular response to DNA damage is the rapid phosphorylation of H2A.X at position Ser139. Cellular location Nucleus Images WB Lysate: MCF-7, HeLa, H1299, JKProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 100 mg/ml nocodazole for 18 h and 100 nM calyculin A for 1 hProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa