anti-histone h2a rabbit pab

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Anti-Histone H2A Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone 2A type 1-B/E, H2AC4, H2AC8, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AFM, HIST1H2AB Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone 2A type 1-B/E, H2AC4, H2AC8, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AFM, HIST1H2AB UniProt ID P04908 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2A protein MW (kDa) 14 Specificity Anti-Histone H2A Rabbit pAb detects endogenous levels of total histone H2A protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Among core histones, the H2A family shows the highest sequence divergence, especially in the C-terminal region, leading to variants including H2A.Z, H2A.X, MacroH2A, H2A.B etc. Histone H2A helps regulate chromatin structure and function through specialized variants and a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, ubiquitination, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Cellular location Nucleus Images WB Lysate: HeLa, COS-7, BRL, mouse liver, mouse kidneyProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-Histone H2A Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone 2A type 1-B/E, H2AC4, H2AC8, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AFM, HIST1H2AB Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone 2A type 1-B/E, H2AC4, H2AC8, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AFM, HIST1H2AB UniProt ID P04908 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H2A protein MW (kDa) 14 Specificity Anti-Histone H2A Rabbit pAb detects endogenous levels of total histone H2A protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Among core histones, the H2A family shows the highest sequence divergence, especially in the C-terminal region, leading to variants including H2A.Z, H2A.X, MacroH2A, H2A.B etc. Histone H2A helps regulate chromatin structure and function through specialized variants and a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, ubiquitination, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Cellular location Nucleus Images WB Lysate: HeLa, COS-7, BRL, mouse liver, mouse kidneyProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 14 kDa Observed band size: 14 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with