Product Name :
anti-his-tag rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
/
UniProt ID :
/
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Cellular location /
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000/1:5000/1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with GAPDH (WT) (36 kDa) expression vector containing a His-tag, whole cell lysateProtein loading quantity: 10 μgExposure time: 10 sObserved band size: 36 kDa ICC/IF Cell line: 293TFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-His-Tag Rabbit mAb Clone Number: JRMR-10094-11 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP Reactivity: / Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / ICC/IF 1:1000 – 1:2000 / IP 1:25 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000/1:5000/1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with GAPDH (WT) (36 kDa) expression vector containing a His-tag, whole cell lysateProtein loading quantity: 10 μgExposure time: 10 sObserved band size: 36 kDa ICC/IF Cell line: 293TFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-His-Tag Rabbit mAb Clone Number: JRMR-10094-11 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP Reactivity: / Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / ICC/IF 1:1000 – 1:2000 / IP 1:25 / Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000/1:5000/1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), 2: HEK-293F transfected with GAPDH (WT) (36 kDa) expression vector containing a His-tag, whole cell lysateProtein loading quantity: 10 μgExposure time: 10 sObserved band size: 36 kDa ICC/IF Cell line: 293TFixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:2000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Sutimlimab medchemexpress
Vadastuximab MedChemExpress
Cy5-conjugated AffiniPure Goat Anti-Mouse IgG H&L: Cy5-conjugated AffiniPure Goat Anti-Mouse IgG H&Lis an -conjugated, goat-derived anti-mouse IgG antibody. Cy5-conjugated AffiniPure Goat Anti-Mouse IgG H&L conjugates the light and heavy chains of mouse IgG antibodies for use in ICC/IF, IHC-F, FC, ELISA experiments in the mouse context.
