anti-acetyl-histone h4 (lys77) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb Clone Number: PA-086-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K77ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K77ac UniProt ID P62805 Immunogen Acetylated human histone H4 (Lys77) peptide MW (kDa) 11 Specificity Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb detects histone H4 only when it is acetylated at Lys77. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, and chromatin dynamics. Recent research has identified that H2BK120ac, H3.3K18ac, and H4K77ac are significantly associated with the survival of hepatocellular carcinoma (HCC) patients. Furthermore, H4K77ac has been linked to HCC recurrence. These findings suggest that H2BK120ac, H3.3K18ac, and H4K77ac may be potential prognostic factors for HCC. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa Lysate: 1: MCF-7; 2: BRL; 3: 20 ng of recombinant histone H4 Protein loading amount: 20 μg for MCF-7 and BRL lysatesBlocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb Clone Number: PA-086-13 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K77ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4K77ac UniProt ID P62805 Immunogen Acetylated human histone H4 (Lys77) peptide MW (kDa) 11 Specificity Anti-Acetyl-Histone H4 (Lys77) Rabbit mAb detects histone H4 only when it is acetylated at Lys77. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 – 1:100 Human ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, and chromatin dynamics. Recent research has identified that H2BK120ac, H3.3K18ac, and H4K77ac are significantly associated with the survival of hepatocellular carcinoma (HCC) patients. Furthermore, H4K77ac has been linked to HCC recurrence. These findings suggest that H2BK120ac, H3.3K18ac, and H4K77ac may be potential prognostic factors for HCC. Cellular location Nucleus Images Dot Blot Peptide amount: 1 ng, 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 30 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours)Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa Lysate: 1: MCF-7; 2: BRL; 3: 20 ng of recombinant histone H4 Protein loading amount: 20 μg for MCF-7 and BRL lysatesBlocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human kidney Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with