anti-hdac6 rabbit mab

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Recombinant Rabbit Monoclonal Anti-HDAC6 Rabbit mAb $ 20% off for full 3 pieces –> Clone Number: JMMR-2618B Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Africangreenmonkey Synonyms: Histone deacetylase 6, HD6, Tubulin-lysine deacetylase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Histone deacetylase 6, HD6, Tubulin-lysine deacetylase UniProt ID Q9UBN7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human, African green monkey IHC-P 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network. It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins. HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP) Lysate: 1: HeLa, 2: COS-7, 3: K562 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 131 kDaObserved MW: 160 kDa IHC-P Tissue: Human kidney Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-HDAC6 Rabbit mAb $ 20% off for full 3 pieces –> Clone Number: JMMR-2618B Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Africangreenmonkey Synonyms: Histone deacetylase 6, HD6, Tubulin-lysine deacetylase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Histone deacetylase 6, HD6, Tubulin-lysine deacetylase UniProt ID Q9UBN7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human, African green monkey IHC-P 1:50-1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network. It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins. HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP) Lysate: 1: HeLa, 2: COS-7, 3: K562 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 131 kDaObserved MW: 160 kDa IHC-P Tissue: Human kidney Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for