anti-golph2 mouse mab

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Recombinant Anti-GOLPH2 Mouse mAb Clone Number: JMMR-3591 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: GOLM1, Golgi membrane protein 1, GP73 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms GOLM1, Golgi membrane protein 1, GP73 UniProt ID Q8NBJ4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Golgi phosphoprotein 2 (GOLPH2), also known as GP73 or GOLM1, is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. GOLPH2 MW is 73 kDa in western blotting due to phosphorylation. Cellular location Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°Covernight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)Lysate: 1: SH-SY 5Y, 2: MDA-MB-231 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 45 kDa Observed band size: 87 kDa IHC-P Tissue: Human liver carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-GOLPH2 Mouse mAb Clone Number: JMMR-3591 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: GOLM1, Golgi membrane protein 1, GP73 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms GOLM1, Golgi membrane protein 1, GP73 UniProt ID Q8NBJ4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Golgi phosphoprotein 2 (GOLPH2), also known as GP73 or GOLM1, is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. GOLPH2 MW is 73 kDa in western blotting due to phosphorylation. Cellular location Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°Covernight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate)Lysate: 1: SH-SY 5Y, 2: MDA-MB-231 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 45 kDa Observed band size: 87 kDa IHC-P Tissue: Human liver carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with