anti-glypican 3 rabbit mab

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Recombinant Rabbit Monoclonal Anti-Glypican 3 Rabbit mAb Clone Number: JRMR-59 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human Synonyms: GTR2-2, MXR7, GPC3, OCI5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms GTR2-2, MXR7, GPC3, OCI5 UniProt ID P51654 Immunogen Recombinant human Glypican 3 fragment MW (kDa) 66 Specificity Anti-Glypican 3 Rabbit mAb recognizes endogeneous level of Glypican 3 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HepG2 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 66 kDa Observed band size: 66 kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Glypican 3 Rabbit mAb Clone Number: JRMR-59 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human Synonyms: GTR2-2, MXR7, GPC3, OCI5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms GTR2-2, MXR7, GPC3, OCI5 UniProt ID P51654 Immunogen Recombinant human Glypican 3 fragment MW (kDa) 66 Specificity Anti-Glypican 3 Rabbit mAb recognizes endogeneous level of Glypican 3 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human FC 1:50 – 1:100 Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. Cellular location Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: HepG2 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 66 kDa Observed band size: 66 kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with