anti-glutaryllysine rabbit pab

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Anti-Glutaryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Kglu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kglu UniProt ID / Immunogen Glutarylated lysine peptides MW (kDa) Multiple Specificity Anti-Glutaryllysine Rabbit pAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions. Cellular location / Images Dot Blot Peptide amount: 1 ng, 5 ng, 25 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: HeLa, MEF, S2, S.cerevisiae, E.coliProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple :
Anti-Glutaryllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: All Synonyms: Kglu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kglu UniProt ID / Immunogen Glutarylated lysine peptides MW (kDa) Multiple Specificity Anti-Glutaryllysine Rabbit pAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions. Cellular location / Images Dot Blot Peptide amount: 1 ng, 5 ng, 25 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides used in the experiment is provided in the table below. WB Lysate: HeLa, MEF, S2, S.cerevisiae, E.coliProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple