anti-glutaryllysine mouse mab

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Anti-Glutaryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: All Synonyms: Kglu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kglu UniProt ID / Immunogen Glutarylated lysine peptides MW (kDa) Multiple Specificity Anti-Glutaryllysine Mouse mAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions. Cellular location / Images Dot Blot Peptide/Protein amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. WB Lysate: HeLa, N2a, BRLProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple ChIP Cell type: HeLa cellsCross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30 exon 3, LDHA, LDHA promoter, FOXO3a promoter, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-Glutaryllysine Mouse mAb Clone Number: / Host: Mouse Clonality: Monoclonal Applications： WB ChIP Reactivity: All Synonyms: Kglu Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kglu UniProt ID / Immunogen Glutarylated lysine peptides MW (kDa) Multiple Specificity Anti-Glutaryllysine Mouse mAb detects proteins post-translationally modified by glutarylation on lysine residues. This pan antibody recognizes glutarylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All ChIP 6 μg per 5×106 cells Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Lysine glutarylation is a histone post-translational modification (PTM) that has been recently identified through integrated proteomic approaches and elaborate biochemistry analyses. This modification has been shown to be evolutionarily conserved across multiple species of prokaryotes and eukaryotes, and is present in a wide range of proteins, including both histones and non-histone substrates. Lysine glutarylation is a PTM that is regulated by SIRT5 and nutrient availability, and it has been demonstrated to impact various metabolic processes and mitochondrial functions. Cellular location / Images Dot Blot Peptide/Protein amount: 1 ng, 4 ng, 16 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of peptides and proteins used in the experiment is provided in the table below. WB Lysate: HeLa, N2a, BRLProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: MultipleObserved band size: Multiple ChIP Cell type: HeLa cellsCross-linking conditions: No cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μgBeads type and amount per IP: 50 μL of Protein A/G MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal mouse IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, RPL30 exon 3, LDHA, LDHA promoter, FOXO3a promoter, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.