anti-frzb mouse mab

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Recombinant Anti-FRZB Mouse mAb Clone Number: JMMR-2704 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: Frezzled, Fritz, FrzB-1, FIZ, FRE, FRP, FRZB1, SFRP3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Frezzled, Fritz, FrzB-1, FIZ, FRE, FRP, FRZB1, SFRP3 UniProt ID Q92765 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background FRZB is a soluble frizzled-related proteins (sFRPS) that function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. FRZB also appears to be involved in limb skeletogenesis. It regulates chondrocyte maturation and long bone development. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: LNCaP, 2: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 35 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 35 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-FRZB Mouse mAb Clone Number: JMMR-2704 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: Frezzled, Fritz, FrzB-1, FIZ, FRE, FRP, FRZB1, SFRP3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Frezzled, Fritz, FrzB-1, FIZ, FRE, FRP, FRZB1, SFRP3 UniProt ID Q92765 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:50 Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background FRZB is a soluble frizzled-related proteins (sFRPS) that function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. FRZB also appears to be involved in limb skeletogenesis. It regulates chondrocyte maturation and long bone development. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: LNCaP, 2: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 35 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Neuro-2a Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 36 kDa Observed band size: 35 kDa ICC/IF Cell line: Neuro-2a Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with