Product Name: Akt1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, IP, WB
Applications: Akt1 antibody can be used for the detection of Akt1 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Akt1 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human Akt1.The immunogen is located within amino acids 20 – 70 of Akt1.
Host Species: Rabbit
Purification: Akt1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 67684-64-4
Product: trans-ACPD
Buffer: Akt1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Akt1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Akt1 Antibody: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Protein kinase B
Accession NO.: AAH00479
Protein Ino: 12653417
Official Symbol: AKT1
Geneid: 207
Background: Akt1 Antibody: Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt, is part of the phosphatidyl 3-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival. Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2. The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad. Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors.
PubMed ID:http://aac.asm.org/content/52/8/2767.abstract
