anti-estrogen receptor alpha rabbit mab

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Recombinant Rabbit Monoclonal Anti-Estrogen Receptor alpha Rabbit mAb Clone Number: MR097C Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: Estrogen receptor, ER-alpha, ESR, NR3A1 Product Size ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Estrogen receptor, ER-alpha, ESR, NR3A1 UniProt ID P03372 Immunogen MW (kDa) 66 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:100 – 1:500 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Estrogen receptor alpha (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains. Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery.Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7. Ser167 may be phosphorylated by p90RSK and Akt. According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients. Cellular location Nucleus, Membrane, Cytoplasm, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate: 1: SK-BR-3 (Negative cell line), 2: MCF-7, 3: T-47DProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 66 kDa Observed band size: 66 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Estrogen Receptor alpha Rabbit mAb Clone Number: MR097C Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: Estrogen receptor, ER-alpha, ESR, NR3A1 Product Size ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Estrogen receptor, ER-alpha, ESR, NR3A1 UniProt ID P03372 Immunogen MW (kDa) 66 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:100 – 1:500 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Estrogen receptor alpha (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains. Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery.Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7. Ser167 may be phosphorylated by p90RSK and Akt. According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients. Cellular location Nucleus, Membrane, Cytoplasm, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate: 1: SK-BR-3 (Negative cell line), 2: MCF-7, 3: T-47DProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 66 kDa Observed band size: 66 kDa IHC-P Tissue: Human breast cancer Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with