anti-esrrb mouse mab

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Recombinant Anti-ESRRB Mouse mAb Clone Number: 13651 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: ERR beta-2, ERR-beta, ERRB2, ESRL2, NR3B2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms ERR beta-2, ERR-beta, ERRB2, ESRL2, NR3B2 UniProt ID O95718 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Estrogen-related receptor beta (ESRRB), also known as NR3B2 (nuclear receptor subfamily 3, group B, member 2), is a nuclear receptor that is encoded by the ESRRB gene in human. ESRRB with sequence similarity to the estrogen receptor, a member of nuclear hormone receptor family of steroid hormone receptors. ESRRB binds a canonical ESRRB recognition (ERRE) sequence 5’TCAAGGTCA-3′ localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: A549, 2: Caco-2, 3: HEK-293, 4: JARProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: HEK-293 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with :
Recombinant Anti-ESRRB Mouse mAb Clone Number: 13651 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: ERR beta-2, ERR-beta, ERRB2, ESRL2, NR3B2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms ERR beta-2, ERR-beta, ERRB2, ESRL2, NR3B2 UniProt ID O95718 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Estrogen-related receptor beta (ESRRB), also known as NR3B2 (nuclear receptor subfamily 3, group B, member 2), is a nuclear receptor that is encoded by the ESRRB gene in human. ESRRB with sequence similarity to the estrogen receptor, a member of nuclear hormone receptor family of steroid hormone receptors. ESRRB binds a canonical ESRRB recognition (ERRE) sequence 5’TCAAGGTCA-3′ localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: A549, 2: Caco-2, 3: HEK-293, 4: JARProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: HEK-293 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with