Product Name :
anti-elp4 rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
C11orf19, PAXNEB
UniProt ID :
Q96EB1
Immunogen:
Recombinant human ELP4 fragment
MW (kDa) :
53, 59
Specificity:
Anti-ELP4 Rabbit mAb recognizes endogeneous level of ELP4 protein.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Elongator Acetyltransferase Complex Subunit 4 (ELP4) is a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. The elongator complex catalyzes formation of carboxy methyluridine in the wobble base at position 34 in tRNAs. Diseases associated with ELP4 include Aniridia 2 and Coloboma of Optic Nerve. Among its related pathways are Mesodermal Commitment Pathway and Chromatin organization. Cellular location Cell nucleus and cytosol
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 47 kDaObserved band size: 53, 59 kDa (two isoforms differ from the canonical sequence) ICC/IF Cell line: HepG2Fixation: 100% methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Comment: Color green is the positive signal for
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-ELP4 Rabbit mAb Clone Number: JRMR-10680-46 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: C11orf19, PAXNEB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms C11orf19, PAXNEB UniProt ID Q96EB1 Immunogen Recombinant human ELP4 fragment MW (kDa) 53, 59 Specificity Anti-ELP4 Rabbit mAb recognizes endogeneous level of ELP4 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Elongator Acetyltransferase Complex Subunit 4 (ELP4) is a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. The elongator complex catalyzes formation of carboxy methyluridine in the wobble base at position 34 in tRNAs. Diseases associated with ELP4 include Aniridia 2 and Coloboma of Optic Nerve. Among its related pathways are Mesodermal Commitment Pathway and Chromatin organization. Cellular location Cell nucleus and cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 47 kDaObserved band size: 53, 59 kDa (two isoforms differ from the canonical sequence) ICC/IF Cell line: HepG2Fixation: 100% methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Comment: Color green is the positive signal for
Recombinant Rabbit Monoclonal Anti-ELP4 Rabbit mAb Clone Number: JRMR-10680-46 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: C11orf19, PAXNEB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms C11orf19, PAXNEB UniProt ID Q96EB1 Immunogen Recombinant human ELP4 fragment MW (kDa) 53, 59 Specificity Anti-ELP4 Rabbit mAb recognizes endogeneous level of ELP4 protein. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Elongator Acetyltransferase Complex Subunit 4 (ELP4) is a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. The elongator complex catalyzes formation of carboxy methyluridine in the wobble base at position 34 in tRNAs. Diseases associated with ELP4 include Aniridia 2 and Coloboma of Optic Nerve. Among its related pathways are Mesodermal Commitment Pathway and Chromatin organization. Cellular location Cell nucleus and cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: NIH-3T3, 3: BRLProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 47 kDaObserved band size: 53, 59 kDa (two isoforms differ from the canonical sequence) ICC/IF Cell line: HepG2Fixation: 100% methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Comment: Color green is the positive signal for
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