anti-dykddddk tag mouse mab (binds to flag® tag sequence)

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Recombinant Anti-DYKDDDDK Tag Mouse mAb (Binds to FLAG® Tag Sequence) Clone Number: 13762 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IP ChIP Reactivity: Human Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / IP 1:25 – 1:100 / Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein’s function. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000/1:5000/1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T transfected with an empty vector (vector control), whole cell lysate ; 2: HEK-293T transfected with PFKFB3 (WT) (58 kDa) expression vector containing a Flag-tag, whole cell lysateProtein loading quantity: 10 μgExposure time: 1 sObserved band size: 58 kDa IP IP of HEK-293T transfected with PFKFB3 (WT) (60 kDa) expression vector containing a Flag-tag cells extractsIP Ab incubation condition: :
Recombinant Anti-DYKDDDDK Tag Mouse mAb (Binds to FLAG® Tag Sequence) Clone Number: 13762 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IP ChIP Reactivity: Human Synonyms: / Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms / UniProt ID / Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 / IP 1:25 – 1:100 / Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The DYKDDDDK epitope tag, also known as the FLAG® tag (registered trademark of Sigma-Aldrich) is an eight-amino acid peptide sequence that can be fused to a protein of interest. This tag allows for the detection and purification of the tagged protein with minimal disruption to the tagged protein’s function. Cellular location / Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000/1:5000/1:10000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HEK-293T transfected with an empty vector (vector control), whole cell lysate ; 2: HEK-293T transfected with PFKFB3 (WT) (58 kDa) expression vector containing a Flag-tag, whole cell lysateProtein loading quantity: 10 μgExposure time: 1 sObserved band size: 58 kDa IP IP of HEK-293T transfected with PFKFB3 (WT) (60 kDa) expression vector containing a Flag-tag cells extractsIP Ab incubation condition: