anti-acetyl-histone h3 (lys27) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PA-133-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human Synonyms: H3K27ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27ac UniProt ID P68431 Immunogen Acetylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the structurally similar crotonylated, hydroxyisobutyrylated, β-hydroxybutyrylated, or butyrylated H3 peptide at Lys27 or Lys9. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys9, Lys14, or Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human IHC-P 1:500 – 1:1000 Human ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Immunogen peptide quantity: 1ng, 4 ng, 16 ng,64 ngExposure time: 30 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa, (+): HeLa+ sodium butyrate (30 mM, 4 hr)Protein loading quantity: 20 μgExposure time: 30 sPredicted MW: 15 kDaObserved MW: 15 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & paraffinembedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Description: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PA-133-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP ChIP Reactivity: Human Synonyms: H3K27ac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27ac UniProt ID P68431 Immunogen Acetylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity Anti-Acetyl-Histone H3 (Lys27) Rabbit mAb detects histone H3 only when it is acetylated at Lys27. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys27, but not the structurally similar crotonylated, hydroxyisobutyrylated, β-hydroxybutyrylated, or butyrylated H3 peptide at Lys27 or Lys9. The antibody does not cross-react with other acetylated H3 peptides at Lys4, Lys9, Lys14, or Lys23. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:3000 Human IHC-P 1:500 – 1:1000 Human ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs), known as the “histone code”, are key mechanisms of epigenetics that modulate chromatin structures. The PTMs on histone including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone acetylation, tightly controlled by the opposing action of histone acetyltransferases (HATs) and histone deacetylases (HDACs), occurs primarily at lysine residues on the N-terminal tails of histones H2A (Lys5, 9, and 15), H2B (Lys5,12, 15, 16, and 20), H3 (Lys4, 9, 14, 18, 23, 27, and 36), and H4 (Lys5, 8, 12, 16, and 20), and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L(HRP)Immunogen peptide quantity: 1ng, 4 ng, 16 ng,64 ngExposure time: 30 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:3000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa, (+): HeLa+ sodium butyrate (30 mM, 4 hr)Protein loading quantity: 20 μgExposure time: 30 sPredicted MW: 15 kDaObserved MW: 15 kDa IHC-P Tissue: Human spleenSection type: Formalin-fixed & paraffinembedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Description: Color brown is the positive signal for