anti-dkk3 mouse mab

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Recombinant Anti-DKK3 Mouse mAb Clone Number: JMMR-2690 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Rat Synonyms: Dickkopf-3, hDkk-3, REIC Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Dickkopf-3, hDkk-3, REIC UniProt ID Q9UBP4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:3000-1:10000 Human, Rat IHC-P 1:50 – 1:200 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Dickkopf-related protein 3(DKK3) is a protein in the Dickkopf family that is encoded by the DKK3 gene in human. DKK3 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: SH-SY5Y, 2: U-87 MG, 3: PC-12, 4: A549 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 55 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 55 kDa IHC-P Tissue: Human cardiac muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 4°C overnight Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-DKK3 Mouse mAb Clone Number: JMMR-2690 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Rat Synonyms: Dickkopf-3, hDkk-3, REIC Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Dickkopf-3, hDkk-3, REIC UniProt ID Q9UBP4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:3000-1:10000 Human, Rat IHC-P 1:50 – 1:200 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Dickkopf-related protein 3(DKK3) is a protein in the Dickkopf family that is encoded by the DKK3 gene in human. DKK3 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development. Cellular location Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: SH-SY5Y, 2: U-87 MG, 3: PC-12, 4: A549 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 55 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: Rat brain Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 55 kDa IHC-P Tissue: Human cardiac muscle Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 4°C overnight Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with