anti-dio2 mouse mab

Vapor Pressure :
Recombinant Anti-DIO2 Mouse mAb Clone Number: 10439 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: 5DII, DIOII, Type 2 DI, ITDI2, TXDI2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms 5DII, DIOII, Type 2 DI, ITDI2, TXDI2 UniProt ID Q92813 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Type II iodothyronine deiodinase (iodothyronine 5′-deiodinase, iodothyronine 5′-monodeiodinase) is an enzyme that in humans is encoded by the DIO2 gene. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3′,5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves’ disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3′ UTR of Sec-containing genes have a common stem-loop structure, the Sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF7, 2: PC-3, 3: SH-SY5Y, 4: A549Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 31 kDaObserved band size: 30-35 kDa IHC-P Tissue: Human thyroid cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-DIO2 Mouse mAb Clone Number: 10439 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: 5DII, DIOII, Type 2 DI, ITDI2, TXDI2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms 5DII, DIOII, Type 2 DI, ITDI2, TXDI2 UniProt ID Q92813 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Type II iodothyronine deiodinase (iodothyronine 5′-deiodinase, iodothyronine 5′-monodeiodinase) is an enzyme that in humans is encoded by the DIO2 gene. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3′,5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves’ disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3′ UTR of Sec-containing genes have a common stem-loop structure, the Sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF7, 2: PC-3, 3: SH-SY5Y, 4: A549Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 31 kDaObserved band size: 30-35 kDa IHC-P Tissue: Human thyroid cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with